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Directional TOPO
Tip: This system helps users design TOPO cloning constructs easily. It covers both Non-directional TA TOPO Cloning method and Directional TOPO Cloning Method.
Vector: Linear Load Sequences:
 
Note: The TA TOPO vector must start with 'A' and end with 'T'; Directional TOPO vector must end with 'CACC'.  
Insert: Linear Circular Load Sequences:
 
Cloning Method: TA-TOPO Cloning Directional TOPO Cloning
Example Sequences